SKU SQK-RNA004
This kit is highly recommended for users who:
Please note that to use this kit, you will need to purchase additional 3rd party reagents: see the “3rd Party Materials” tab for more detail.
| Feature | Property |
|---|---|
| Preparation time | 135 minutes |
| Input requirement | 300 ng poly(A)+ RNA or 1 μg total RNA |
| PCR required | No |
| Fragmentation | No |
| Read length | RNA length |
| Associated protocols | • Direct RNA sequencing (SQK-RNA004) |
| Pack size | 6 reactions |
| Stability | Shipped at 2–8°C
Long-term storage -20 – -30°C The warranty for this product is 3 months from receipt by the customer |
The Direct RNA Sequencing Kit (SQK-RNA004) is used to prepare and sequence native RNA without conversion to cDNA. Inputs include poly(A)-tailed RNA or total RNA, such as eukaryotic mRNA and viral RNA.
This kit upgrade includes increased sequencing output and improved modal raw read accuracy on our new RNA flow cell (FLO-MIN004RA and FLO-PRO004RA). The flow cell priming reagents are also included as part of the sequencing kit and have been reformulated to be compatible on the new RNA flow cell. Other updates include fuel fix technology, allowing users to run longer experiments without the need for fuel addition during the run.
In applications where Direct RNA sequencing’s key advantages (detection of modified bases and reduced bias) are not important, users may consider one of Oxford Nanopore’s cDNA kits which currently provide the highest output per run.
Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
The Direct RNA Sequencing Kit (SQK-RNA004) is used to prepare either poly(A)-tailed RNA or total RNA for sequencing by performing reverse transcription to synthesise a secondary complementary cDNA strand for stability to improve sequencing output. Sequencing adapters are then attached to the RNA-cDNA hybrid for sequencing.
• Direct RNA sequencing (SQK-RNA004)
Safety Data Sheet for Direct RNA Sequencing Kit (SQK-RNA004)
Note: We have increased the concentration of the RNA CS (RCS) vials found in newer batches of SQK-RNA004. Please ensure you are following the correct method and inputs for your RCS concentration:
| Batch RNA004.20.xxxx or older | Batch RNA004.30.0001 or newer |
|---|---|
| Lower concentration of the RNA CS (RCS) vial:
15 ng/µl |
Increased concentration of the RNA CS (RCS) vial:
50 ng/µl |
The Direct RNA Sequencing Kit contains enough reagents for six reactions.
| Name | Acronym | Cap colour | No. of vials | Fill volume per vial (μl) |
|---|---|---|---|---|
| RT Adapter | RTA | Blue | 1 | 10 |
| RNA Ligation Adapter | RLA | Green | 1 | 45 |
| RNA CS | RCS | Yellow | 1 | 25 |
| Wash Buffer | WSB | Orange | 2 | 1,200 |
| RNA Elution Buffer | REB | Black | 1 | 300 |
| Library Solution | LIS | White cap, pink label | 1 | 600 |
| Sequencing Buffer | SB | Red | 1 | 700 |
| RNA Flush Tether | RFT | Pink | 1 | 200 |
| Flow Cell Flush | FCF | Clear cap, light blue label | 1 | 8,000 |
Note: The RNA CS (RCS) is the calibration strand and contains the Enolase II from YHR174W, extracted from the yeast saccharomyces cerevisiae. The reference FASTA files for the yeast is available here.
Note: The above are 3rd party reagents required for the standard prep. For other workflows using this kit, extra 3rd party reagents may be required.
The Direct RNA Sequencing Kit can be used together with:
Note: The Flow Cell Wash Kit (EXP-WSH004) can be used to remove library between flow cell washes and library re-loading. Please note, this kit is not compatible with RNA as a nuclease wash.
